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  • Strains

pTrcA*H

Ancestors
  • All plasmids
    • pTrc99A
      • pTrcA*H



Main features
Ptrc-metAfbr(A881G)-yjeH

Selection marker:

Ampicillin



Modifications
Gene Organism Modification
metA
Escherichia coli
Feedback insensitive
yjeH
Escherichia coli
Overexpression



Strains harboring plasmid pTrcA*H
  • Escherichia coli W3110 ΔmetJ (pTrcA*H)
  • Escherichia coli W3110 ΔmetJΔmetI (pTrcA*H)
  • Escherichia coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H
  • Escherichia coli W3110 ΔmetJΔmetIΔlysAΔthrB/pTrcA*H
  • Escherichia coli W3110 ΔmetJΔmetIΔthrB/pTrcA*H

References
  • Jian‐Feng Huang, Zhi‐Qiang Liu, Li‐Qun Jin, Xiao‐Ling Tang, Zhen‐Yang Shen, Huan‐Huan Yin & Yu‐Guo Zheng (2016). Metabolic engineering of Escherichia coli for microbial production of L‐methionine. Biotechnology & Bioengineering.

Reference

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