pETDuet-1

Ancestors

Derivated

pCTSDT

pET-pgi

pET-pgm

pET-glmM

pET-glmU

pET-glmS

pET-glmUS

pET-glmM-glmUS

pET-galE

pET-galT

pET-galK

pET-galET

galETK

pET-galETK-futC

pETDuet-fdh

Main features

pBR322 origin, P_T7lac-RBS, P_T7lac-RBS, two MCS

Selection marker:

Ampicillin

References

Li Zhou, Ying Zhu, Zhongzhe Yuan, Guangqing Liu, Zijin Sun, Shiyu Du, He Liu, Yating Li, Haili Liu, and Zhemin Zhou (2022). Evaluation of Metabolic Engineering Strategies on 2-Ketoisovalerate Production by Escherichia coli. Applied and Environmental Microbiology. 88(17): e00976-22.

Hu, Miaomiao; Li, Mengli; Li, Chenchen & Zhang, Tao. Biosynthesis of Lacto-N-fucopentaose I in Escherichia coli by metabolic pathway rational design. Carbohydrate Polymers. 2022, 297, 120017.

Zheng, Zhaojuan; Zhao, Mingyue; Zang, Ying; Zhou, Ying & Ouyang, Jia. Production of optically pure l-phenyllactic acid by using engineered Escherichia coli coexpressing l-lactate dehydrogenase and formate dehydrogenase. Journal of Biotechnology. 2015, 207, 47-51.

Ke, Qin; Liu, Chang; Zhuang, Yibin; Xue, Yaju; Cui, Zhanzhao; Zhang, Cuiying; Yin, Hua & Liu, Tao. Metabolic engineering of Escherichia coli for high-level production of benzyl acetate from glucose. Microbial Cell Factories. 2024, 23(1).