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  • Strains

pBRthrABCygaZH

Ancestors
  • All plasmids
    • pBR322
      • pBRThrABC
        • pBRthrABCygaZH



Main features
thrAfbrBC ygaZH

Selection marker:

Ampicillin



Modifications
Gene Organism Modification
thrA
Escherichia coli
Feedback insensitive
thrB
Escherichia coli
Overexpression
thrC
Escherichia coli
Overexpression
ygaZ
Escherichia coli
Overexpression
ygaH
Escherichia coli
Overexpression



Strains harboring plasmid pBRthrABCygaZH

Escherichia coli ILE01 (pTacilvAIH, pBRthrABCygaZH)

Escherichia coli ILE02 (pTacilvAIH, pBRthrABCygaZH)

Escherichia coli ILE03 (pTacilvAIH, pBRthrABCygaZH)


References

Jin Hwan Park, Jae Eun Oh, Kwang Ho Lee, Ji Young Kim & Sang Yup Lee (2012). Rational Design of Escherichia coli for l‑Isoleucine Production. ACS Synthetic Biology.


Reference

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