pPs619C1310-CpPCT540

Ancestors

Main features

phaC1Ps6-19 variant (phaC1310Ps6-19; E130D, S477F, Q481K), pctCp variant (pct540Cp; V193A, four silent nucleotide mutations of T78C, T669C, A1125G, T1158C)

Selection marker:

Ampicillin

Modifications

Gene	Organism	Modification
*PhaC1*	Pseudomonas sp. MBEL 6-19	Overexpression
*pct*	Clostridium propionicum	Overexpression

References

Yu Kyung Jung, Tae Yong Kim, Si Jae Park & Sang Yup Lee (2009). Metabolic engineering of Escherichia coli for the production of polylactic acid and its copolymers. Biotechnology & Bioengineering.