pTrc99a-Bspck-pncB

Ancestors

All plasmids

pTrc99A

pTrc99a-Bspck

pTrc99a-Bspck-pncB

Main features

pck_Bs, pncB

Selection marker:

Ampicillin

Modifications

Gene	Organism	Modification
*pckA*	Bacillus subtilis	Overexpression
*pncB*	Escherichia coli	Overexpression

Strains harboring plasmid pTrc99a-Bspck-pncB

Escherichia coli BA062

References

Jiang, Youming; Zheng, Tianwen; Ye, Xiaohan; Xin, Fengxue; Zhang, Wenming; Dong, Weiliang; Ma, Jiangfeng & Jiang, Min. Metabolic engineering of Escherichia coli for l-malate production anaerobically. Microbial Cell Factories. 2020, 19(1).