pCDFDuet-1

Ancestors

Derivated

pCDF-Duet-serAmut-serC

pJNU-4

Main features

CloDF13 ori, lacI, pT7lac, pT7lac

Selection marker:

Streptomycin

Spectinomycin

References

Hemanshu Mundhada, Konstantin Schneider, Hanne Bjerre Christensen & Alex Toftgaard Nielsen (2015). Engineering of high yield production of L‐serine in Escherichia coli. Biotechnology & Bioengineering.

Yu Deng, Ning Ma, Kangjia Zhu, Yin Mao, Xuetuan Wei & Yunying Zhao (2018). Balancing the carbon flux distributions between the TCA cycle and glyoxylate shunt to produce glycolate at high yield and titer in Escherichia coli. Metabolic Engineering.