pCDFDuet-1
Ancestors
Derivated
CloDF13 ori, lacI, PT7lac-lgtA, pT7lac-wbgO
Main features
CloDF13 ori, lacI, pT7lac, pT7lac, two MCSSelection marker:
Streptomycin
Spectinomycin
References
Hemanshu Mundhada, Konstantin Schneider, Hanne Bjerre Christensen & Alex Toftgaard Nielsen (2015). Engineering of high yield production of L‐serine in Escherichia coli. Biotechnology & Bioengineering.
Yu Deng, Ning Ma, Kangjia Zhu, Yin Mao, Xuetuan Wei & Yunying Zhao (2018). Balancing the carbon flux distributions between the TCA cycle and glyoxylate shunt to produce glycolate at high yield and titer in Escherichia coli. Metabolic Engineering.
Hu, Miaomiao; Li, Mengli; Li, Chenchen & Zhang, Tao. Biosynthesis of Lacto-N-fucopentaose I in Escherichia coli by metabolic pathway rational design. Carbohydrate Polymers. 2022, 297, 120017.