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pS4

Ancestors
  • All plasmids
    • pBbB5c
      • pS4



Main features
pBBR1 ori, aroE-aroD-aroBop-PLtetO-1-aroG*-ppsA-tktA

Selection marker:

Chloramphenicol



Modifications
Gene Organism Modification
aroE
Escherichia coli
Overexpression
aroD
Escherichia coli
Overexpression
aroB
Escherichia coli
Overexpression
aroG
Escherichia coli
Feedback insensitive
ppsA
Escherichia coli
Overexpression
tktA
Escherichia coli
Overexpression



Strains harboring plasmid pS4

Escherichia coli C (pS4, pY1)

Escherichia coli E (pS4, pY2)

Escherichia coli F (pS4, pY3)


References

Darmawi Juminaga, Edward E. K. Baidoo, Alyssa M. Redding-Johanson, Tanveer S. Batth, Helcio Burd, Aindrila Mukhopadhyay, Christopher J. Petzold & Jay D. Keasling (2011). Modular Engineering of l-Tyrosine Production in Escherichia coli. Applied and Environmental Microbiology.


Reference

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