pLS01

Ancestors

Main features
pSC101 replicon, Ptrc ΔlacIq ΔlacO-synRBS-ribA-synRBS-ribB-synRBS-ribD-synRBS-ribE-synRBS-ribC

Selection marker:

Chloramphenicol



Modifications
Gene Organism Modification
Overexpression
Overexpression
Overexpression
Overexpression
Overexpression



Strains harboring plasmid pLS01

Escherichia coli LS32T

Escherichia coli LS33T

Escherichia coli LS34T

Escherichia coli LS35T

Escherichia coli LS36T

Escherichia coli LS37T

Escherichia coli LS38T

Escherichia coli LS39T

Escherichia coli LS40T

Escherichia coli LS41T

Escherichia coli LS42T

Escherichia coli LS43T

Escherichia coli LS44T

Escherichia coli LS45T

Escherichia coli LS47T

Escherichia coli LS49T

Escherichia coli LS50T

Escherichia coli LS51T

Escherichia coli LS52T

Escherichia coli LS53T

Escherichia coli LS54T

Escherichia coli LS55T

Escherichia coli LS56T

Escherichia coli LS69T

Escherichia coli LS70T

Escherichia coli LS71T

Escherichia coli RF01T

Escherichia coli RF03T

Escherichia coli LS01T

Escherichia coli LS31T

Escherichia coli LS46T

Escherichia coli LS48T

Escherichia coli LS02T


References

Dai, Jiu; Geng, Mingjie; Du, Yong; Iqbal, Muhammad Waleed; Yang, Haoyu; Shen, Xiaolin; Wang, Jia; Sun, Xinxiao & Yuan, Qipeng. Microbial Synthesis of Nucleosides: Advances and Prospects. ACS Synthetic Biology. 2025, 14(1), 1-9.

Liu, Shuang; Kang, Pei; Cui, Zhenzhen; Wang, Zhiwen & Chen, Tao. Increased riboflavin production by knockout of 6-phosphofructokinase I and blocking the Entner–Doudoroff pathway in Escherichia coli. Biotechnology Letters. 2016, 38(8), 1307-1314.