p20C-EC10

Ancestors

Main features
pBR322 replicon, Ptrc-synRBS-ribA-synRBS-ribB-synRBS-ribD-synRBS-ribE-synRBS-ribC

Selection marker:

Ampicillin



Modifications
Gene Organism Modification
Overexpression
Overexpression
Overexpression
Overexpression
Overexpression



Strains harboring plasmid p20C-EC10

Escherichia coli RF01S

Escherichia coli RF03S

Escherichia coli RF05S-M40


References

Liu, Shuang; Kang, Pei; Cui, Zhenzhen; Wang, Zhiwen & Chen, Tao. Increased riboflavin production by knockout of 6-phosphofructokinase I and blocking the Entner–Doudoroff pathway in Escherichia coli. Biotechnology Letters. 2016, 38(8), 1307-1314.