p20C-EC10

Ancestors

All plasmids

p20C

p20C-EC10

Main features

pBR322 replicon, P_trc-synRBS-ribA-synRBS-ribB-synRBS-ribD-synRBS-ribE-synRBS-ribC

Selection marker:

Ampicillin

Modifications

Gene	Organism	Modification
*ribA*	Escherichia coli	Overexpression
*ribB*	Escherichia coli	Overexpression
*ribD*	Escherichia coli	Overexpression
*ribE*	Escherichia coli	Overexpression
*ribC*	Escherichia coli	Overexpression

Strains harboring plasmid p20C-EC10

Escherichia coli RF05S-M40

Escherichia coli RF01S

Escherichia coli RF02S

Escherichia coli RF03S

Escherichia coli RF05S

Escherichia coli RF01S/p15Trc-zg

Escherichia coli RF01S/p15Trc

Escherichia coli RF01S/p15Trc-zgp

References

Liu, Shuang; Kang, Pei; Cui, Zhenzhen; Wang, Zhiwen & Chen, Tao. Increased riboflavin production by knockout of 6-phosphofructokinase I and blocking the Entner–Doudoroff pathway in Escherichia coli. Biotechnology Letters. 2016, 38(8), 1307-1314.

Lin, Zhenquan; Xu, Zhibo; Li, Yifan; Wang, Zhiwen; Chen, Tao & Zhao, Xueming. Metabolic engineering of Escherichia coli for the production of riboflavin. Microbial Cell Factories. 2014, 13(1).