CloDF13 ori, lacI, P_T7lac-lgtA, p_T7lac-wbgO

Ancestors

All plasmids

pCDFDuet-1

CloDF13 ori, lacI, P_T7lac-lgtA, p_T7lac-wbgO

Selection marker:

Chloramphenicol

Streptomycin

Modifications

Gene	Organism	Modification
*lgtA*	Neisseria meningitidis	Overexpression
*wbgO*	Escherichia coli	Overexpression

References

Hu, Miaomiao; Li, Mengli; Li, Chenchen & Zhang, Tao. Biosynthesis of Lacto-N-fucopentaose I in Escherichia coli by metabolic pathway rational design. Carbohydrate Polymers. 2022, 297, 120017.