pET-galETK-futC

Ancestors

All plasmids

pETDuet-1

pET-galETK-futC

Main features

pBR322 replicon, P_T7-RBS-galETK, P_T7-RBS-futC

Selection marker:

Ampicillin

Modifications

Gene	Organism	Modification
*galE*	Escherichia coli	Overexpression
*galT*	Escherichia coli	Overexpression
*galK*	Escherichia coli	Overexpression
*futC*	Helicobacter pylori	Overexpression

References

Hu, Miaomiao; Li, Mengli; Li, Chenchen & Zhang, Tao. Biosynthesis of Lacto-N-fucopentaose I in Escherichia coli by metabolic pathway rational design. Carbohydrate Polymers. 2022, 297, 120017.