pTrc-TBSgEMX

Ancestors

All plasmids

pTrc99A

pTrc-TPH

pTrc-TB

pTrc-TBE

pTrc-TBEM

pTrc-TBEMX

pTrc-TBSgEMX

Main features

tph_Dj, phhB_Pe, folE_Sg, folM, folX

Selection marker:

Ampicillin

Modifications

Gene	Organism	Modification
*tph*	Dugesia japonica	Overexpression
*phhB*	Pseudomonas aeruginosa	Overexpression
*folM*	Escherichia coli	Overexpression
*folX*	Escherichia coli	Overexpression
*folE*	Streptomyces griseus	Overexpression

Strains harboring plasmid pTrc-TBSgEMX

Escherichia coli HTP-15

References

Li, Qingchen; Li, Chenxi; Zhong, Jie; Wang, Yukun; Yang, Qinghua; Wang, Bingmei; He, Wenjin; Huang, Jianzhong; Lin, Shengyuan & Qi, Feng. Metabolic engineering of Escherichia coli for N-methylserotonin biosynthesis. Metabolic Engineering. 2025, 87, 49-59.