pAC-TBBsEMX

Ancestors

All plasmids

pACYC184

pAC-TBBsEMX

Main features

tph_Dj, phhB_Pe, folE_Bs, folM, folX

Selection marker:

Chloramphenicol

Modifications

Gene	Organism	Modification
*tph*	Dugesia japonica	Overexpression
*phhB*	Pseudomonas aeruginosa	Overexpression
*folEA*	Bacillus subtilis	Overexpression
*folM*	Escherichia coli	Overexpression
*folX*	Escherichia coli	Overexpression

Strains harboring plasmid pAC-TBBsEMX

Escherichia coli NMS-20

References

Li, Qingchen; Li, Chenxi; Zhong, Jie; Wang, Yukun; Yang, Qinghua; Wang, Bingmei; He, Wenjin; Huang, Jianzhong; Lin, Shengyuan & Qi, Feng. Metabolic engineering of Escherichia coli for N-methylserotonin biosynthesis. Metabolic Engineering. 2025, 87, 49-59.