pBR322-P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA

Ancestors

All plasmids

pBR322

pBR322-P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA

Derivated

pBR322-P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A

Main features

P_tac-aroG^S211F -P_tac-trpE^{Q71K/S94N/C465Y} -trpABCD-serA, lacI

Selection marker:

Tetracycline

Modifications

Gene	Organism	Modification
*aroG*	Escherichia coli	Feedback insensitive
*trpE*	Escherichia coli	Feedback insensitive
*trpD*	Escherichia coli	Overexpression
*trpC*	Escherichia coli	Overexpression
*trpB*	Escherichia coli	Overexpression
*trpA*	Escherichia coli	Overexpression
*serA*	Escherichia coli	Overexpression
*lacI*	Escherichia coli	Overexpression

Strains harboring plasmid pBR322-P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA

Escherichia coli TS

References

Hou, Minglei; Gao, Shengqi; Wu, Jing; Chen, Sheng & Zhang, Kang. Metabolic engineering of Escherichia coli to enhance L-tryptophan biosynthesis. Systems Microbiology and Biomanufacturing. 2025.