pBR322-P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A

Ancestors

All plasmids

pBR322

pBR322-P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA

pBR322-P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A

Derivated

pBR322-P_tac-aroG^S211F-P_tac-trpE^S40F-trpABCD-serA^H344A/N364A

pBR322-P_tac-aroG^S211F-P_tac−trpE^A63V-trpABCD-serA^H344A/N364A

Main features

P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A

Selection marker:

Tetracycline

Modifications

Gene	Organism	Modification
*aroG*	Escherichia coli	Feedback insensitive
*trpE*	Escherichia coli	Feedback insensitive
*trpD*	Escherichia coli	Overexpression
*trpC*	Escherichia coli	Overexpression
*trpB*	Escherichia coli	Overexpression
*trpA*	Escherichia coli	Overexpression
*serA*	Escherichia coli	Feedback insensitive

Strains harboring plasmid pBR322-P_tac-aroG^S211F-P_tac-trpE^{Q71K/S94N/C465Y}-trpABCD-serA^H344A/N364A

Escherichia coli TS-1

Escherichia coli TS-1A

Escherichia coli TS-1B

References

Hou, Minglei; Gao, Shengqi; Wu, Jing; Chen, Sheng & Zhang, Kang. Metabolic engineering of Escherichia coli to enhance L-tryptophan biosynthesis. Systems Microbiology and Biomanufacturing. 2025.