pJBEI-6409

Ancestors

All plasmids

pJBEI-6409

Main features

P_UV5-AtoB-saHMGS-saHMGR-T1ter-P_trc-MK-PMK-PMD-idi-T_B0015-P_trc-trGPPS-tLS-p15Aori-cam^R-lacI

Selection marker:

Chloramphenicol

Modifications

Gene	Organism	Modification
*atoB*	Escherichia coli	Overexpression
*ERG13*	Saccharomyces cerevisiae	Overexpression
*ERG1*	Saccharomyces cerevisiae	Overexpression
*ERG12*	Saccharomyces cerevisiae	Overexpression
*ERG8*	Saccharomyces cerevisiae	Overexpression
*MVD1*	Saccharomyces cerevisiae	Overexpression
*idi*	Escherichia coli	Overexpression
*GPPS2*	Abies grandis	Overexpression
LS	Mentha spicata	Overexpression
*lacI*	Escherichia coli	Overexpression

Strains harboring plasmid pJBEI-6409

Escherichia coli EcoCTs03

Escherichia coli HK overexpressed

Escherichia coli LDH KO

Escherichia coli ALDH-ADH KO

References

Khanijou, Jasmeet Kaur; Hee, Yan Ting; Scipion, Clement P. M.; Chen, Xixian & Selvarajoo, Kumar. Systems biology approach for enhancing limonene yield by re-engineering Escherichia coli. npj Systems Biology and Applications. 2024, 10(1).