pTrc99a-leuA^fbr-cgb

Ancestors

All plasmids

pTrc99A

pTrc99a-leuA^fbr-cgb

Main features

leuA^fbr_Cg

Selection marker:

Ampicillin

Modifications

Gene	Organism	Modification
*leuA*	Corynebacterium glutamicum	Feedback insensitive

Strains harboring plasmid pTrc99a-leuA^fbr-cgb

Escherichia coli LEU01

References

Hao, Yanan; Pan, Xuewei; Li, Guomin; You, Jiajia; Zhang, Hengwei; Yan, Sihan; Xu, Meijuan & Rao, Zhiming. Construction of a plasmid-free l-leucine overproducing Escherichia coli strain through reprogramming of the metabolic flux. Biotechnology for Biofuels and Bioproducts. 2023, 16(1).