pAP01

Ancestors


Derivated

pAP11

pAP12

pAP13

pAP31

pAP22

pAP21


Main features
pAP with aroGfbr and tyrAfbr, p15A ori with PRPL

Selection marker:

Kanamycin



Modifications
Gene Organism Modification
Feedback insensitive
Feedback insensitive



Strains harboring plasmid pAP01

Escherichia coli WSH-Z06 (pAP01)

Escherichia coli WSH-Z06 (pAP01) ΔldhA

Escherichia coli WSH-Z06 (pAP01) ΔpflB

Escherichia coli WSH-Z06 (pAP01) ΔadhE

Escherichia coli WSH-Z06 (pAP01) ΔtrpE

Escherichia coli WSH-Z06 (pAP01) ΔpheA

Escherichia coli WSH-Z06 (pAP01) ΔtyrR

Escherichia coli WSH-Z06 (pAP01) ΔtyrR,pheA

Escherichia coli WSH-Z06 (pAP01) ΔtyrR,trpE

Escherichia coli WSH-Z06 (pAP01) ΔtyrR,adhE

Escherichia coli WSH-Z06 (pAP01) ΔtyrR,pflB

Escherichia coli WSH-Z06 (pAP01) ΔtyrR,ldhA

Escherichia coli WSH-Z06 (pAP01) ΔtyrR,pheA,trpE

Escherichia coli WSH-Z06 (pAP01) ΔtyrR,pheA,adhE

Escherichia coli WSH-Z06 (pAP01) ΔtyrR,pheA,pflB

Escherichia coli WSH-Z06 (pAP01) ΔtyrR,pheA,ldhA


References

Ping, Jurong; Wang, Lian; Qin, Zhijie; Zhou, Zhemin & Zhou, Jingwen. Synergetic engineering of Escherichia coli for efficient production of l-tyrosine. Synthetic and Systems Biotechnology. 2023, 8(4), 724-731.