pAP21

Ancestors

All plasmids

pAP-B03

pAP01

pAP21

Main features

pAP with aroG^fbr, tyrA^fbr, tktA, ppsA and aroABC, p15A ori with P_RP_L

Selection marker:

Kanamycin

Modifications

Gene	Organism	Modification
*aroG*	Escherichia coli	Feedback insensitive
*tyrA*	Escherichia coli	Feedback insensitive
*tktA*	Escherichia coli	Overexpression
*ppsA*	Escherichia coli	Overexpression
*aroA*	Escherichia coli	Overexpression
*aroB*	Escherichia coli	Overexpression
*aroC*	Escherichia coli	Overexpression

Strains harboring plasmid pAP21

Escherichia coli WSH-Z06 (pAP21)

References

Ping, Jurong; Wang, Lian; Qin, Zhijie; Zhou, Zhemin & Zhou, Jingwen. Synergetic engineering of Escherichia coli for efficient production of l-tyrosine. Synthetic and Systems Biotechnology. 2023, 8(4), 724-731.