pSTV-Plac-T7rbs-davBA-gabT-yqhD

Ancestors

All plasmids

pACYC184

pSTV28

pSTV-Plac-T7rbs-davBA-gabT-yqhD

Main features

P_lac-RBS-davBA-RBS-gabT-RBS-yqhD

Selection marker:

Chloramphenicol

Modifications

Gene	Organism	Modification
*davB*	Pseudomonas putida	Overexpression
*davA*	Pseudomonas putida	Overexpression
*gabT*	Escherichia coli	Overexpression
*yqhD*	Escherichia coli	Overexpression

Strains harboring plasmid pSTV-Plac-T7rbs-davBA-gabT-yqhD

Escherichia coli 5HV1

Escherichia coli 5HV4

Escherichia coli 5HV5

Escherichia coli 5HV6

References

Chen, Chen; Wang, Tiantian; Ye, Pan & Li, Naiqiang. Metabolic engineering of Escherichia coli for the efficient production of 5-hydroxyvaleric acid. Process Biochemistry. 2023, 130, 625-633.