pBRmetL–rhtA

Ancestors

All plasmids

pBR322

pBRmetL

pBRmetL–rhtA

Main features

metL, P_rhtA-rhtA

Selection marker:

Tetracycline

Chloramphenicol

Modifications

Gene	Organism	Modification
*metL*	Escherichia coli	Overexpression
*rhtA*	Escherichia coli	Overexpression

Strains harboring plasmid pBRmetL–rhtA

Escherichia coli HM4 (pBRmetL–rhtA)

References

Li, Hua; Wang, Baoshi; Zhu, Linghuan; Cheng, Shi; Li, Youran; Zhang, Liang; Ding, Zhong Yang; Gu, Zheng Hua & Shi, Gui Yang. Metabolic engineering of Escherichia coli W3110 for L-homoserine production. Process Biochemistry. 2016, 51(12), 1973-1983.