pACHTP-LMT

Ancestors

All plasmids

pACHTP-LMT

Main features

p15A ori, Lac_pro-mtrA-PTPS-SPR, M1-93_pro-PCD-DHPR, T7_pro-TPH145

Selection marker:

Chloramphenicol

Modifications

Gene	Organism	Modification
*folEA*	Bacillus subtilis	Overexpression
*PTPS*	Homo sapiens	Overexpression
*SPR*	Homo sapiens	Overexpression
*PCBD*	Homo sapiens	Overexpression
*DHPR*	Homo sapiens	Overexpression
*TPH2*	Homo sapiens	Overexpression

Strains harboring plasmid pACHTP-LMT

Escherichia coli HTP101-LMT

Escherichia coli BL21Δtna/pACHTP-LMT

Escherichia coli TRP0/pACHTP-LMT

References

Xu, Da; Fang, Mengjun; Wang, Haijiao; Huang, Lei; Xu, Qinyang & Xu, Zhinan. Enhanced production of 5-hydroxytryptophan through the regulation of L-tryptophan biosynthetic pathway. Applied Microbiology and Biotechnology. 2020, 104(6), 2481-2488.