pET-30a_m-rtxA-D5

Ancestors

All plasmids

pET-30a(+)

pET-30a_m-rtxA-D5

Main features

pBR322 ori, P_T7-lacO-rtxA-D5 (rtxA-D2 and 463 bp upstream of rtxA)

Selection marker:

Kanamycin

Modifications

Gene	Organism	Modification
*rtxA*	Bradyrhizobium elkanii	Overexpression

References

Yuchang Luo, Qinqin Zhao, Qian Liu & Yan Feng (2019). An Artificial Biosynthetic Pathway for 2‑Amino-1,3-Propanediol Production Using Metabolically Engineered Escherichia coli. ACS Synthetic Biology.