pNLSt1

Ancestors

All plasmids

pRSFDuet-1

pNLSt1

Main features

P_T7-MsLS-P_T7-SltNPPS (Truncate the first 45 residues)

Selection marker:

Kanamycin

Modifications

Gene	Organism	Modification
LS	Mentha spicata	Overexpression
*NDPS1*	Solanum lycopersicum	Overexpression

References

Dengwei Lei, Zetian Qiu, Jihua Wu, Bin Qiao, Jianjun Qiao & Guang-Rong Zhao (2021). Combining Metabolic and Monoterpene Synthase Engineering for de Novo Production of Monoterpene Alcohols in Escherichia coli. ACS Synthetic Biology.