pNO1

Ancestors

All plasmids

pRSFDuet-1

pNPPS

pNO1

Main features

P_T7-GmNES-P_T7-SltNPPS (truncated)

Selection marker:

Kanamycin

Modifications

Gene	Organism	Modification
*NES*	Glycine max	Overexpression
*NDPS1*	Solanum lycopersicum	Overexpression

Strains harboring plasmid pNO1

Escherichia coli ENO1

References

Dengwei Lei, Zetian Qiu, Jihua Wu, Bin Qiao, Jianjun Qiao & Guang-Rong Zhao (2021). Combining Metabolic and Monoterpene Synthase Engineering for de Novo Production of Monoterpene Alcohols in Escherichia coli. ACS Synthetic Biology.