pMevΔFPPS

Ancestors

All plasmids

pMev

pMevΔFPPS

Main features

P_lacUV5-atoB-HMGS-tHMGR-MK-PMK-PMDidi

Selection marker:

Chloramphenicol

Modifications

Gene	Organism	Modification
*atoB*	Escherichia coli	Overexpression
*ERG13*	Saccharomyces cerevisiae	Overexpression
*HMG1*	Saccharomyces cerevisiae	Overexpression
*ERG12*	Saccharomyces cerevisiae	Overexpression
*ERG8*	Saccharomyces cerevisiae	Overexpression
*MVD1*	Saccharomyces cerevisiae	Overexpression
*idi*	Escherichia coli	Overexpression

Strains harboring plasmid pMevΔFPPS

Escherichia coli 010H (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A)

Escherichia coli 040H (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A)

Escherichia coli 050H (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A)

Escherichia coli 060HA (pMevΔFPPS/pET-FPPS-(PT)₄P-PTS2C415F/H454A)

References

Zhou, Li; Wang, Yuxi; Han, Laichuang; Wang, Qin; Liu, Haili; Cheng, Ping; Li, Ruoxuan; Guo, Xuecong & Zhou, Zhemin. Enhancement of Patchoulol Production in Escherichia coli via Multiple Engineering Strategies. Journal of Agricultural and Food Chemistry. 2021, 69(27), 7572-7580.