pET-FPPS-(PT)₄P-PTS2C415F

Ancestors

All plasmids

pET-28a(+)

pET-PTS2

pET-FPPS-PTS2

pET-FPPS-(PT)₄P-PTS2

pET-FPPS-(PT)₄P-PTS2C415F

Derivated

pET-FPPS-(PT)₄P-PTS2C415F/H454A

Main features

ispA-(PT)₄P linker-PTS2^C415F

Selection marker:

Kanamycin

Modifications

Gene	Organism	Modification
*ispA*	Escherichia coli	Overexpression
*PTS2*	Pogostemon cablin	Overexpression

References

Zhou, Li; Wang, Yuxi; Han, Laichuang; Wang, Qin; Liu, Haili; Cheng, Ping; Li, Ruoxuan; Guo, Xuecong & Zhou, Zhemin. Enhancement of Patchoulol Production in Escherichia coli via Multiple Engineering Strategies. Journal of Agricultural and Food Chemistry. 2021, 69(27), 7572-7580.