pDG35

Ancestors

All plasmids

pBBR1MCS

pDG35

Main features

P_T7: aroG^fbr, trpE^fbr, and serA^fbr

Selection marker:

Chloramphenicol

Modifications

Gene	Organism	Modification
*aroG*	Escherichia coli	Feedback insensitive
*trpE*	Escherichia coli	Feedback insensitive
*serA*	Escherichia coli	Feedback insensitive

Strains harboring plasmid pDG35

Escherichia coli DG122

References

Daoyi Guo, Sijia Kong, Xu Chu, Xun Li & Hong Pan (2019). De Novo Biosynthesis of Indole-3-acetic Acid in Engineered Escherichia coli. Journal of Agricultural & Food Chemistry.