pMEL03

Ancestors

All plasmids

pACYC184

pSTV28

pMEL03

Derivated

Main features

ppsA tktA

Selection marker:

Chloramphenicol

Modifications

Gene	Organism	Modification
*tktA*	Escherichia coli	Overexpression
*ppsA*	Escherichia coli	Overexpression

Strains harboring plasmid pMEL03

Escherichia coli TRTH1013

Escherichia coli Dmtr

Escherichia coli Dpta

References

Jian Wang, Li-Kun Cheng, Jing Wang, Qian Liu, Tong Shen & Ning Chen (2013). Genetic engineering of Escherichia coli to enhance production of l-tryptophan. Applied Microbiology and Biotechnology.

Tong Shen, Qing Liu, Xixian Xie, Qingyang Xu & Ning Chen (2012). Improved Production of Tryptophan in Genetically Engineered Escherichia coli with TktA and PpsA Overexpression. BioMed Research International.