Escherichia coli LS34T

Ancestors

Genotype with respect to parental

P_trc-purF::ldhA

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔpfkA, Δedd, Δeda | Plasmid pLS01 added | P_trc-fbp | ΔpurR | P_trc-purF::ldhA

Bars (|) indicate differences between strains.

Plasmids

• pLS01 (pSC101 replicon, P_trc ΔlacI^q ΔlacO-synRBS-ribA-synRBS-ribB-synRBS-ribD-synRBS-ribE-synRBS-ribC)

References

• Dai, Jiu; Geng, Mingjie; Du, Yong; Iqbal, Muhammad Waleed; Yang, Haoyu; Shen, Xiaolin; Wang, Jia; Sun, Xinxiao & Yuan, Qipeng. Microbial Synthesis of Nucleosides: Advances and Prospects. ACS Synthetic Biology. 2025, 14(1), 1-9.