Escherichia coli LS46T

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli LS02

Escherichia coli LS02T

Escherichia coli LS46T

Genotype with respect to parental

P_trc-prs-purF::ldhA, P_trc-gmk-ndk::ybeM

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔpfkA, Δedd, Δeda | Plasmid pLS01 added | P_trc-prs-purF::ldhA, P_trc-gmk-ndk::ybeM

Bars (|) indicate differences between strains.

Plasmids

pLS01 (pSC101 replicon, P_trc ΔlacI^q ΔlacO-synRBS-ribA-synRBS-ribB-synRBS-ribD-synRBS-ribE-synRBS-ribC)

Modifications

Gene	Organism	Modification
ldhA	Escherichia coli	Silencing
prsA	Escherichia coli	Overexpression
purF	Escherichia coli	Overexpression
ybeM	Escherichia coli	Silencing
gmk	Escherichia coli	Overexpression
ndk	Escherichia coli	Overexpression

References

Dai, Jiu; Geng, Mingjie; Du, Yong; Iqbal, Muhammad Waleed; Yang, Haoyu; Shen, Xiaolin; Wang, Jia; Sun, Xinxiao & Yuan, Qipeng. Microbial Synthesis of Nucleosides: Advances and Prospects. ACS Synthetic Biology. 2025, 14(1), 1-9.