Escherichia coli RF01S

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli RF01S

Derived strains

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid p20C-EC10 added

Bars (|) indicate differences between strains.

Plasmids

p20C-EC10 (pBR322 replicon, P_trc-synRBS-ribA-synRBS-ribB-synRBS-ribD-synRBS-ribE-synRBS-ribC)

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
riboflavin		Titer	220.0 mg/L		Glucose		Flask	[ 247 ]
riboflavin		Titer	229.1 mg/L		Glucose		Flask	[ 248 ]

* Inferred from plots using RetroPlot.

References

Liu, Shuang; Kang, Pei; Cui, Zhenzhen; Wang, Zhiwen & Chen, Tao. Increased riboflavin production by knockout of 6-phosphofructokinase I and blocking the Entner–Doudoroff pathway in Escherichia coli. Biotechnology Letters. 2016, 38(8), 1307-1314.

Lin, Zhenquan; Xu, Zhibo; Li, Yifan; Wang, Zhiwen; Chen, Tao & Zhao, Xueming. Metabolic engineering of Escherichia coli for the production of riboflavin. Microbial Cell Factories. 2014, 13(1).