Escherichia coli RF03T

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Δpgi, Δedd, Δeda | Plasmid pLS01 added

Bars (|) indicate differences between strains.


Plasmids

  • pLS01 (pSC101 replicon, Ptrc ΔlacIq ΔlacO-synRBS-ribA-synRBS-ribB-synRBS-ribD-synRBS-ribE-synRBS-ribC)


Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
riboflavin Titer 516.0 mg/L Glucose 23 h Flask [ 247 ]
riboflavin Titer 502.0 mg/L 4.0 g/L Glucose Flask [ 247 ]
riboflavin Substrate yield 50.2 mg/g of sustrate 4.0 g/L Glucose Flask [ 247 ]
riboflavin Specific production rate 5.5 mg/g DCW/h 4.0 g/L Glucose Flask [ 247 ]
riboflavin Titer 540.0 mg/L 4.1 g/L Glucose Flask [ 247 ]
riboflavin Substrate yield 54.1 mg/g of sustrate 4.1 g/L Glucose Flask [ 247 ]
riboflavin Specific production rate 5.7 mg/g DCW/h 4.1 g/L Glucose Flask [ 247 ]
riboflavin Titer 570.0 mg/L 4.2 g/L Glucose Flask [ 247 ]
riboflavin Substrate yield 57.2 mg/g of sustrate 4.2 g/L Glucose Flask [ 247 ]
riboflavin Specific production rate 5.9 mg/g DCW/h 4.2 g/L Glucose Flask [ 247 ]
riboflavin Titer 597.0 mg/L 4.5 g/L Glucose Flask [ 247 ]
riboflavin Substrate yield 59.6 mg/g of sustrate 4.5 g/L Glucose Flask [ 247 ]
riboflavin Specific production rate 5.8 mg/g DCW/h 4.5 g/L Glucose Flask [ 247 ]

* Inferred from plots using RetroPlot.


References