Escherichia coli RF05S-M40
Ancestors
Genotype with respect to parental
Ptrc-acs, ribF-RBS*
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Δpgi | Δedd, Δeda | Plasmid p20C-EC10 added | Ptrc-acs, ribF-RBS*Bars (|) indicate differences between strains.
Plasmids
-
p20C-EC10 (pBR322 replicon, Ptrc-synRBS-ribA-synRBS-ribB-synRBS-ribD-synRBS-ribE-synRBS-ribC)
Modifications
| Gene | Organism | Modification |
|---|---|---|
|
Overexpression
|
||
|
Attenuation
|
Production
| Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|---|
| riboflavin | Titer | 2702.8 mg/L | Glucose | Flask | [ 247 , 248 ] | |||
| riboflavin | Substrate yield | 137.5 mg/g of sustrate | Glucose | Flask | [ 247 ] | |||
| riboflavin | Titer | 1036.1 mg/L | Glucose | Flask | [ 248 ] | |||
| riboflavin | Titer | 1507.0 mg/L | Glucose | Flask | [ 248 ] |
* Inferred from plots using RetroPlot.