Escherichia coli RF05S-M40

Ancestors
Genotype with respect to parental

Ptrc-acs, ribF-RBS*


Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Δpgi, Δedd, Δeda | Plasmid p20C-EC10 added | Ptrc-acs, ribF-RBS*

Bars (|) indicate differences between strains.


Plasmids
  • p20C-EC10 (pBR322 replicon, Ptrc-synRBS-ribA-synRBS-ribB-synRBS-ribD-synRBS-ribE-synRBS-ribC)


Modifications
Gene Organism Modification
acs
Overexpression
Attenuation

Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
riboflavin Titer 2.7 g/L Glucose Flask [ 247 ]
riboflavin Substrate yield 0.13 g/g of sustrate Glucose Flask [ 247 ]

* Inferred from plots using RetroPlot.


References