Escherichia coli LS02T

Ancestors

Derived strains

Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔpfkA, Δedd, Δeda | Plasmid pLS01 added

Bars (|) indicate differences between strains.


Plasmids

  • pLS01 (pSC101 replicon, Ptrc ΔlacIq ΔlacO-synRBS-ribA-synRBS-ribB-synRBS-ribD-synRBS-ribE-synRBS-ribC)


Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
riboflavin Titer 667.0 mg/L 3.8 g/L Glucose Flask [ 247 ]
riboflavin Titer 10.4 g/L 32.0 g/L Glucose 71 h Fed-Batch [ 247 ]
riboflavin Substrate yield 56.8 mg/g of sustrate 32.0 g/L Glucose 71 h Fed-Batch [ 247 ]
riboflavin Titer 5.8 g/L Glucose Fed-Batch [ 247 ]
riboflavin Substrate yield 67.9 mg/g of sustrate 3.8 g/L Glucose Flask [ 247 ]
riboflavin Specific production rate 7.8 mg/g DCW/h 3.8 g/L Glucose Flask [ 247 ]
riboflavin Titer 605.0 mg/L 3.9 g/L Glucose Flask [ 247 ]
riboflavin Substrate yield 61.7 mg/g of sustrate 3.9 g/L Glucose Flask [ 247 ]
riboflavin Specific production rate 6.7 mg/g of sustrate 3.9 g/L Glucose Flask [ 247 ]
riboflavin Titer 551.0 mg/L 3.9 g/L Glucose Flask [ 247 ]
riboflavin Substrate yield 53.4 mg/g of sustrate 3.9 g/L Glucose Flask [ 247 ]
riboflavin Specific production rate 6.1 mg/g DCW/h 3.9 g/L Glucose Flask [ 247 ]
riboflavin Titer 327.0 mg/L 4.2 g/L Glucose Flask [ 247 ]
riboflavin Substrate yield 31.8 mg/g of sustrate 4.2 g/L Glucose Flask [ 247 ]
riboflavin Specific production rate 3.4 mg/g DCW/h 4.2 g/L Glucose Flask [ 247 ]
riboflavin Titer 296.0 mg/L 4.4 g/L Glucose Flask [ 247 ]
riboflavin Substrate yield 26.8 mg/g of sustrate 4.4 g/L Glucose Flask [ 247 ]
riboflavin Specific production rate 2.9 mg/g DCW/h 4.4 g/L Glucose Flask [ 247 ]

* Inferred from plots using RetroPlot.


References