Escherichia coli RF01S/p15Trc-zgp

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid p20C-EC10 added | Plasmid p15Trc-zgp added

Bars (|) indicate differences between strains.


Plasmids

  • p20C-EC10 (pBR322 replicon, Ptrc-synRBS-ribA-synRBS-ribB-synRBS-ribD-synRBS-ribE-synRBS-ribC)

    p15Trc-zgp (p15A replication, Ptrc-synRBS-zwf-synRBS-gnd-synRBS-pgl, zwf and gnd genes from C. glutamicum, pgl gene from E. coli)


Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
riboflavin Titer 228.4 mg/L 3.1 OD600 Glucose Flask [ 248 ]
riboflavin Titer 31.9 mg/g of sustrate 3.1 OD600 Glucose Flask [ 248 ]

* Inferred from plots using RetroPlot.


References