Escherichia coli MG-P8

Ancestors

All strains

Escherichia coli K12

Escherichia coli CGSC4510

Escherichia coli MG-P1

Escherichia coli MG-P8

Genotype with respect to parental

ΔtrpE

Genotype with respect to wild type

F⁺ (λ) | Hfr (PO1), λ−, el4-, tyrA4, relA1, spoT1, thiE1 | ΔtyrB::P_tac-Lappr | Plasmid pTA06 added | ΔtrpE

Bars (|) indicate differences between strains.

Plasmids

pTA06 (P_tac-aroG^fbr-pheA^fbr, P_tac-tktA, ColE1 ori)

Modifications

Gene	Organism	Modification
trpE	Escherichia coli	Silencing

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-(-)-3-Phenyllactic acid		Titer	1.14 g/L	5.22 g/L^*	Glucose		Flask	[ 250 ]

* Inferred from plots using RetroPlot.

References

Wu, Weibin; Chen, Maosen; Li, Chenxi; Zhong, Jie; Xie, Rusheng; Pan, Zhibin; Lin, Junhan & Qi, Feng. Efficient production of phenyllactic acid in Escherichia coli via metabolic engineering and fermentation optimization strategies. Frontiers in Microbiology. 2024, 15.