Escherichia coli MG-P10

Ancestors

Genotype with respect to parental

ΔptsH-ptsI-crr::glf-glk


Genotype with respect to wild type
F+ (λ) | Hfr (PO1), λ−, el4-, tyrA4, relA1, spoT1, thiE1 | ΔtyrB::Ptac-Lappr | Plasmid pTA07 added | ΔtrpE | ΔptsH-ptsI-crr::glf-glk

Bars (|) indicate differences between strains.


Plasmids

  • pTA07 (Ptac-aroGfbr-pheAfbr, Ptac-tktA-pckA, ColE1 ori)


Modifications
Gene Organism Modification
Silencing
Silencing
crr
Silencing
glf
Overexpression
glk
Overexpression

Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
L-(-)-3-Phenyllactic acid Titer 1.42 g/L 5.43 g/L* Glucose 48 h Flask [ 250 ]
L-(-)-3-Phenyllactic acid Titer 52.89 g/L 48.32 g/L Glucose 48 h Fed-Batch [ 250 ]
L-(-)-3-Phenyllactic acid Substrate yield 0.177 g/g of sustrate 56.89 g/L Glucose 48 h Fed-Batch [ 250 ]
L-(-)-3-Phenyllactic acid Titer 45.68 g/L 56.89 g/L Glucose 48 h Fed-Batch [ 250 ]
L-(-)-3-Phenyllactic acid Substrate yield 0.225 g/g of sustrate 48.32 g/L Glucose 48 h Fed-Batch [ 250 ]
acetate Titer 0.81 g/L 56.89 g/L Glucose 48 h Fed-Batch [ 250 ]
L-(-)-3-Phenyllactic acid Titer 31.01 g/L 32.56 g/L Glucose 48 h Fed-Batch [ 250 ]
L-(-)-3-Phenyllactic acid Substrate yield 0.181 g/g of sustrate 32.56 g/L Glucose 48 h Fed-Batch [ 250 ]
L-(-)-3-Phenyllactic acid Volumetric productivity 0.646 g/L/h 32.56 g/L Glucose 48 h Fed-Batch [ 250 ]
acetate Titer 4.5 g/L 32.56 g/L Glucose 48 h Fed-Batch [ 250 ]
L-(-)-3-Phenyllactic acid Volumetric productivity 1.102 g/L/h 48.32 g/L Glucose 48 h Fed-Batch [ 250 ]
L-(-)-3-Phenyllactic acid Volumetric productivity 0.951 g/L/h 56.89 g/L Glucose 48 h Fed-Batch [ 250 ]
L-(-)-3-Phenyllactic acid Titer 49.68 g/L 42.11 g/L Glucose 48 h Fed-Batch [ 250 ]
L-(-)-3-Phenyllactic acid Substrate yield 0.205 g/g of sustrate 42.11 g/L Glucose 48 h Fed-Batch [ 250 ]
L-(-)-3-Phenyllactic acid Volumetric productivity 1.035 g/L/h 42.11 g/L Glucose 48 h Fed-Batch [ 250 ]
acetate Titer 2.68 g/L 42.11 g/L Glucose 48 h Fed-Batch [ 250 ]
acetate Titer 1.98 g/L 48.32 g/L Glucose 48 h Fed-Batch [ 251 ]

* Inferred from plots using RetroPlot.


References