Escherichia coli ET05

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli ET01

Escherichia coli ET03

Escherichia coli ET05

Genotype with respect to parental

ΔlysA, ΔthrA

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pTrc-ectABC added | ΔlysA | ΔlysA, ΔthrA

Bars (|) indicate differences between strains.

Plasmids

pTrc-ectABC (ectABC_Hv)

Modifications

Gene	Organism	Modification
thrA	Escherichia coli	Silencing

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
ectoine	Titer	0.22 g/L	7.1 g/L	Glucose		Flask	[253]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Hao Zhang, Zhong Liang, Ming Zhao, Yanqin Ma, Zhengshan Luo, Sha Li & Hong Xu (2022). Metabolic Engineering of Escherichia coli for Ectoine Production With a Fermentation Strategy of Supplementing the Amino Donor. Frontiers in Bioengineering and Biotechnology.