Escherichia coli ET11

Ancestors

Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pTrc-ectABC added | ΔlysA | ΔpykF | Plasmid pTrc-ectABC lost | Plasmid pTETB added

Bars (|) indicate differences between strains.


Plasmids

  • pTETB (Ptrc-ectABCHv-Ptrc-ectBHv)


Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
ectoine
Titer 12.9 g/L 17.54 g/L* Glucose 48 h * Flask [ 253 ]
ectoine
Titer 53.2 g/L 26.9 g/L* Glucose 48 h * Fed-Batch [ 253 ]
ectoine
Biomass-specific yield 0.74 g/g DCW 17.54 g/L* Glucose 48 h * Flask [ 253 ]
ectoine
Volumetric productivity 1.11 g/L/h 26.9 g/L* Glucose 48 h Fed-Batch [ 253 ]
ectoine
Substrate yield 0.33 g/g of sustrate 26.9 g/L* Glucose 48 h Fed-Batch [ 253 ]
ectoine
Biomass-specific yield 2.0 g/g DCW 26.9 g/L* Glucose 48 h Fed-Batch [ 253 ]

* Inferred from plots using RetroPlot.


References