Escherichia coli IAA68

Ancestors

Genotype with respect to wild type

F−, endA1, hsdR17 (r K −, m K + ), supE44, thi-l, λ−, recA1, gyrA96, ΔlacU169 (Φ80lacZ ΔM15) | ΔtnaA::kan | Δkan | Plasmid pCIA41 added | Plasmid pCLTID23 added

Bars (|) indicate differences between strains.

Plasmids

pCIA41 (P_tac-ipdC_Ec, P_sod-aspC)

pCLTID23 (P_tac-iad1_Um)

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
(indol-3-yl)acetate	Titer	1.8 g/L	4.68 OD₆₀₀^*	L-tryptophan	48 h	Flask	[254]
(indol-3-yl)acetate	Titer	3.0 g/L		L-tryptophan	24 h	Flask	[254]
indole-3-ethanol	Titer	0.032 g/L	4.68 OD₆₀₀^*	L-tryptophan	48 h	Flask	[254]
indole-3-ethanol	Substrate yield	1.05 mol/mol of substrate	4.68 OD₆₀₀^*	L-tryptophan	48 h	Flask	[254]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Elisa Friska Romasi & Jinho Lee (2013). Development of Indole-3-Acetic Acid-Producing Escherichia coli by Functional Expression of IpdC, AspC, and Iad1. Journal of microbiology and biotechnology.