Escherichia coli HS26

Ancestors

Genotype with respect to parental

Crp ^{R83F (CGT-TTC), G142P (GGC-CCC)}

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔJIB* ΔthrB ΔmetA ΔlysA ΔiclR ΔptsG ΔgalR ΔlacI::Ptrc-rhtA Ptrc-rhtA Ptrc-eamA Ptrc-metL Ptrc-thrA Ptrc-glk Ptrc-gltB | Δadd Δamn | P_trc-ado1_Sc | Δyjit::P_trc-thrA^fbr | P_trc::P_asd | ΔgapC::P_trc-aspC | ΔgapC::P_trc-aspC | Crp ^{R83F (CGT-TTC), G142P (GGC-CCC)}

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
crp	Escherichia coli	Unstated

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-homoserine		Titer	13.21 g/L ^*	8.45 OD₆₀₀^*	Glucose		Flask	[ 255 ]

* Inferred from plots using RetroPlot.

References

Niu, Kun; Zheng, Rui; Zhang, Miao; Chen, Mao‐Qin; Kong, Yi‐Ming; Liu, Zhi‐Qiang & Zheng, Yu‐Guo. Adjustment of the main biosynthesis modules to enhance the production of l‐homoserine in Escherichia coli W3110. Biotechnology and Bioengineering. 2024.