Escherichia coli HX4-2

Synonyms

HY42

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli HX1

Escherichia coli HX2

Escherichia coli HX3-2

Escherichia coli HX4-2

Derived strains

Escherichia coli HX5

Genotype with respect to parental

yeep::P_trc- prs_Ec^D128A

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ∆xdhABC | ∆purR | ygay:: P_trc-purF_Bs^{D293V.K316Q.S400W} | yeep::P_trc- prs_Ec^D128A

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
prsA	Escherichia coli	Feedback insensitive

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
hypoxanthine		Titer	0.53 g/L	44.87 OD₆₀₀^*	Glucose		Flask	[ 256 ]

* Inferred from plots using RetroPlot.

References

Siyu Zhao, Tangen Shi, Liangwen Li, Zhichao Chen, Changgeng Li, Zichen Yu, Pengjie Sun & Qingyang Xu (2024). The metabolic engineering of Escherichia coli for the high-yield production of hypoxanthine. Microbial Cell Factories.