Escherichia coli Y1-2

Ancestors

Genotype with respect to parental

ΔpurA::purA_Bs^P242N

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ∆xdhABC | ∆purR | ygay:: P_trc-purF_Bs^{D293V.K316Q.S400W} | yeep::P_trc- prs_Ec^D128A | ilvg::P_trc- purFDNTSQLMKECB_Bs | ΔpurA::purA_Bs^P242N

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
purA	Escherichia coli	Silencing
purA	Bacillus subtilis	Attenuation

Production

Metabolites	Production type	Production	Biomass	Carbon source	Scale	Ref.
hypoxanthine	Titer	0.82 g/L	40.0 OD₆₀₀	Glucose	Flask	[256]
AMP	Titer	0.19 g/L	40.0 OD₆₀₀	Glucose	Flask	[256]
hypoxanthine	Titer	1.38 g/L	47.0 OD₆₀₀	Glucose	Flask	[256]
guanine	Titer	0.07 g/L ^*	40.0 OD₆₀₀	Glucose	Flask	[256]
guanine	Titer	0.13 g/L ^*	47.0 OD₆₀₀	Glucose	Flask	[256]
xanthine	Titer	0.11 g/L ^*	40.0 OD₆₀₀	Glucose	Flask	[256]
xanthine	Titer	0.2 g/L ^*	47.0 OD₆₀₀	Glucose	Flask	[256]
AMP	Titer	0.17 g/L ^*	47.0 OD₆₀₀^*	Glucose	Flask	[256]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Siyu Zhao, Tangen Shi, Liangwen Li, Zhichao Chen, Changgeng Li, Zichen Yu, Pengjie Sun & Qingyang Xu (2024). The metabolic engineering of Escherichia coli for the high-yield production of hypoxanthine. Microbial Cell Factories.