Escherichia coli Y4-1

Ancestors

Genotype with respect to parental

mbhA::P_trc-ushA, gapC::P_trc-nagD, arpB::P_trc-surE

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ∆xdhABC | ∆purR | ygay:: P_trc-purF_Bs^{D293V.K316Q.S400W} | yeep::P_trc- prs_Ec^D128A | ilvg::P_trc- purFDNTSQLMKECB_Bs | ycgh:: P_trc- add_Ec, yjiT::P_trc-ade_Ec | mbhA::P_trc-ushA, gapC::P_trc-nagD, arpB::P_trc-surE

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
ushA	Escherichia coli	Overexpression
gapC	Escherichia coli	Silencing
umpH	Escherichia coli	Overexpression
umpG	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Scale	Ref.
hypoxanthine	Titer	1.95 g/L	47.0 OD₆₀₀^*	Glucose	Flask	[ 256 ]
xanthine	Titer	0.37 g/L	47.0 OD₆₀₀^*	Glucose	Flask	[ 256 ]
guanine	Titer	0.26 g/L	47.0 OD₆₀₀^*	Glucose	Flask	[ 256 ]
AMP	Titer	0.45 g/L ^*	47.0 OD₆₀₀^*	Glucose	Flask	[ 256 ]
hypoxanthine	Substrate yield	0.03 g/g of sustrate ^*	47.0 OD₆₀₀^*	Glucose	Flask	[ 256 ]

* Inferred from plots using RetroPlot.

References

Siyu Zhao, Tangen Shi, Liangwen Li, Zhichao Chen, Changgeng Li, Zichen Yu, Pengjie Sun & Qingyang Xu (2024). The metabolic engineering of Escherichia coli for the high-yield production of hypoxanthine. Microbial Cell Factories.