Escherichia coli Y5-2

Ancestors

Derived strains

Escherichia coli Y5-3

Genotype with respect to parental

rph::P_apFAB104-EsaR

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ∆xdhABC | ∆purR | ygay:: P_trc-purF_Bs^{D293V.K316Q.S400W} | yeep::P_trc- prs_Ec^D128A | ilvg::P_trc- purFDNTSQLMKECB_Bs | ycgh:: P_trc- add_Ec, yjiT::P_trc-ade_Ec | ylbe::P_trc-isn1_Sc | ∆hpt | ∆guaB | rph::P_apFAB104-EsaR

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
rph	Escherichia coli	Silencing
esaR	Pantoea stewartii	Overexpression

References

Siyu Zhao, Tangen Shi, Liangwen Li, Zhichao Chen, Changgeng Li, Zichen Yu, Pengjie Sun & Qingyang Xu (2024). The metabolic engineering of Escherichia coli for the high-yield production of hypoxanthine. Microbial Cell Factories.