Escherichia coli Y6

Ancestors

Derived strains

Escherichia coli Y6-1

Genotype with respect to parental

yjip::P_trc-pur(FDNTSQLMKECB)

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ∆xdhABC | ∆purR | ygay:: P_trc-purF_Bs^{D293V.K316Q.S400W} | yeep::P_trc- prs_Ec^D128A | ilvg::P_trc- purFDNTSQLMKECB_Bs | ycgh:: P_trc- add_Ec, yjiT::P_trc-ade_Ec | ylbe::P_trc-isn1_Sc | ∆hpt | ∆guaB | rph::P_apFAB104-EsaR | yghe::P_esaS-guaB | ycdn:: P_{BBa_j 23110}esaI | yjip::P_trc-pur(FDNTSQLMKECB)

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
purF	Bacillus subtilis	Overexpression
purD	Bacillus subtilis	Overexpression
purN	Bacillus subtilis	Overexpression
purT	Bacillus subtilis	Overexpression
purS	Bacillus subtilis	Overexpression
purQ	Bacillus subtilis	Overexpression
purL	Bacillus subtilis	Overexpression
purM	Bacillus subtilis	Overexpression
purK	Bacillus subtilis	Overexpression
purE	Bacillus subtilis	Overexpression
purC	Bacillus subtilis	Overexpression
purB	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
hypoxanthine	Titer	3.3 g/L ^*	47.0 OD₆₀₀^*	Glucose		Flask	[256]
hypoxanthine	Substrate yield	0.065 g/g of sustrate ^*	47.0 OD₆₀₀^*	Glucose		Flask	[256]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Siyu Zhao, Tangen Shi, Liangwen Li, Zhichao Chen, Changgeng Li, Zichen Yu, Pengjie Sun & Qingyang Xu (2024). The metabolic engineering of Escherichia coli for the high-yield production of hypoxanthine. Microbial Cell Factories.