Escherichia coli Y6-4

Ancestors

Genotype with respect to parental

ynci:: P_{BBa_j 23109}-thrA

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ∆xdhABC | ∆purR | ygay:: P_trc-purF_Bs^{D293V.K316Q.S400W} | yeep::P_trc- prs_Ec^D128A | ilvg::P_trc- purFDNTSQLMKECB_Bs | ycgh:: P_trc- add_Ec, yjiT::P_trc-ade_Ec | ylbe::P_trc-isn1_Sc | ∆hpt | ∆guaB | rph::P_apFAB104-EsaR | yghe::P_esaS-guaB | ycdn:: P_{BBa_j 23110}esaI | yjip::P_trc-pur(FDNTSQLMKECB) | yeel::P_trc- glnA_Bs^{L159I, E304A} | yjgx::P_lac-aspC | ynci:: P_{BBa_j 23109}-thrA

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
thrA	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
hypoxanthine	Titer	3.67 g/L ^*	42.0 g/L^*	Glucose		Flask	[256]
hypoxanthine	Substrate yield	0.062 g/g of sustrate ^*	42.0 OD₆₀₀^*	Glucose		Flask	[256]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Siyu Zhao, Tangen Shi, Liangwen Li, Zhichao Chen, Changgeng Li, Zichen Yu, Pengjie Sun & Qingyang Xu (2024). The metabolic engineering of Escherichia coli for the high-yield production of hypoxanthine. Microbial Cell Factories.