Escherichia coli NMS-19
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔtnaA | ΔqueD | ΔmetJ | Plasmid pAC-DHsI*mlS added | Plasmid pTrc-TBBsEMX addedBars (|) indicate differences between strains.
Plasmids
-
pTrc-TBBsEMX (tphDj, phhBPe, folEBs, folM, folX)
pAC-DHsI*mlS (ddcSs, mtnEc, luxSEc, SAM2Sc)
Production
Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
---|---|---|---|---|---|---|---|---|
N-Methylserotonin |
|
Titer | 128.6 mg/L | L-tryptophan | Fed-Batch | [ 257 ] | ||
N-Methylserotonin |
|
Titer | 5.18 mg/L | L-tryptophan | 12 h | Flask | [ 257 ] | |
5-hydroxy-L-tryptophan |
|
Titer | 252.0 mg/L | L-tryptophan | 84 h | Fed-Batch | [ 257 ] | |
5-hydroxy-L-tryptophan |
|
Titer | 371.5 mg/L | L-tryptophan | 72 h | Fed-Batch | [ 257 ] | |
serotonin |
|
Titer | 20.41 mg/L * | L-tryptophan | Flask | [ 257 ] | ||
5-hydroxy-L-tryptophan |
|
Titer | 23.53 mg/L * | L-tryptophan | Flask | [ 257 ] |
* Inferred from plots using RetroPlot.