Escherichia coli NMS-20

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli MGΔtnaA

Escherichia coli MGΔtnaAΔqueD

Escherichia coli MGΔtnaAΔqueDΔmetJ

Escherichia coli NMS-20

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔtnaA | ΔqueD | ΔmetJ | Plasmid pAC-TBBsEMX added | Plasmid pTrc-DHsI*mlS added

Bars (|) indicate differences between strains.

Plasmids

pTrc-DHsI*mlS (ddc_Ss, mtn_Ec, luxS_Ec, SAM2_Sc)

pAC-TBBsEMX (tph_Dj, phhB_Pe, folE_Bs, folM, folX)

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
N-Methylserotonin		Titer	4.21 mg/L ^*		Glucose		Flask	[ 257 ]
serotonin		Titer	9.9 mg/L ^*		Glucose		Flask	[ 257 ]
5-hydroxy-L-tryptophan		Titer	12.53 mg/L ^*		Glucose		Flask	[ 257 ]

* Inferred from plots using RetroPlot.

References

Li, Qingchen; Li, Chenxi; Zhong, Jie; Wang, Yukun; Yang, Qinghua; Wang, Bingmei; He, Wenjin; Huang, Jianzhong; Lin, Shengyuan & Qi, Feng. Metabolic engineering of Escherichia coli for N-methylserotonin biosynthesis. Metabolic Engineering. 2025, 87, 49-59.